As laboratory professionals, we understand that precision is crucial in diagnostics. One of the key tests we perform is the ALT (Alanine Aminotransferase) test, which plays a vital role in assessing liver health. In this guide, we will walk you through the step-by-step process of conducting an ALT test using a chemistry analyzer, ensuring you achieve accurate results every time. Additionally, we’ll introduce you to Labex International’s high-quality ALT testing reagents and analyzers, designed to enhance your lab's efficiency.
What is ALT and Why is it Important?
Alanine aminotransferase (ALT) is an enzyme primarily found in the liver. Elevated levels of ALT can signal liver damage, making this test essential for diagnosing conditions such as hepatitis or cirrhosis. By accurately measuring ALT levels, healthcare providers can effectively monitor liver health, making it a standard procedure in clinical laboratories.
At Labex International, we offer top-notch reagents and advanced chemistry analyzers to help laboratories like yours perform reliable ALT tests with ease. Explore our ALT reagent kits and automated analyzers to elevate your lab's capabilities.
Reagent Preparation: Ensuring Accuracy with Labex Reagents
Proper reagent preparation is critical for obtaining accurate results in an ALT test. Here’s how to prepare your reagents using Chemistry kits:
1. ALT Reagents
R1 (Buffer): Contains Tris buffer (pH 7.8), L-alanine, and lactate dehydrogenase (LDH).
R2 (Substrate): Contains NADH and α-ketoglutarate.
2. Preparing the Working Reagent (WR)
To create the working reagent, mix 4 parts(eg. 400 uL) of R1 with 1 part(eg. 100 uL) of R2. Labex reagents are formulated for a long shelf life—21 days at 2-8°C or 72 hours at room temperature. By using Labex ALT reagent kits, you ensure optimal stability and accuracy in your tests, suitable for both manual and automated chemistry analyzers.
Step-by-Step ALT Test Procedure Using Labex Chemistry Analyzers
With the right setup and equipment, performing ALT tests can be straightforward. Follow these steps for accurate results:
Assay Conditions
Method | : | Kinetic |
Wavelength | : | 340 nm |
Temperature | : | 37°C |
Delay/Lag time | : | 20 Seconds |
Measuring time | : | 60 Seconds |
Sample Volume | : | 450 uL |
Unit | : | U/L |
Factor | : | 1746 |
Reagent Blank | : | Not Required |
Sample Blank | : | Not Required |
Standard | : | Not Required |
Linear Range | : | Upto 800 |
1. Zeroing the Analyzer
Begin by zeroing your analyzer using distilled water.
2. Pipetting Reagents and Sample
Add 500 uL of Working Reagent into the tube.
Add 50 µL of the patient sample (serum or plasma) into the tube.
Mix thoroughly and sip the Reagent/sample mixture to the analyzer by pressing the sipper button.
3. Measurement
The reaction is kinetic, and the analyzer will measure absorbance over time. You will get the auto calculated result as concentration in uL. However, if you want to Record the absorbance manually, You should read absorbance every minute for 3 minutes. Automated and Semi automated chemistry analyzers simplify this process, minimizing human error and ensuring reliable results.
Quality Control: Ensuring Accuracy with Control Sera
Quality control (QC) is essential for verifying the reliability of your ALT test results. Labex International provides CONTROL NORMAL and CONTROL PATHOLOGICAL sera to help monitor your assay’s performance.
How to Run Quality Control with Labex Control Sera
1. Run Control Sera Daily
Pipette 500 uL of working reagent and 50 µL of control serum (normal or pathological) into the plastic tube.
Mix and read the control/reagent mixture to analyzer same as you did with sample. Just make sure that you run the control by selecting the control option from the Test type selection menu.
2. Interpret the Results
Compare your QC results to the expected reference range. Each control has its own value and you should have one with your control vial. If the values fall within range, you can proceed with patient testing. For optimal results, use multi CALIBRATOR to ensure accurate calibration of your equipment.
Why Choose Labex International for ALT Testing?
At Labex International, we are dedicated to providing high-quality reagents and innovative chemistry analyzers that help laboratories achieve precision and efficiency. Whether you need ALT reagent kits or automated analyzers, we have everything you need to streamline your workflow and deliver reliable diagnostic results.
Our Products:
ALT Reagent Kits: For accurate and stable ALT enzyme measurement.
Automated Chemistry Analyzers: Reduce manual handling and increase throughput.
Control Sera: Ensure reliable quality control results with multiCONTROL NORMAL and PATHOLOGICAL sera.
Calibrators: Maintain accuracy with multiCALIBRATOR.
Conclusion: Trust Labex International for Reliable ALT Testing
Conducting an ALT test is a crucial diagnostic tool for evaluating liver health. With Labex International's high-quality reagents, analyzers, and control sera, you can ensure your results are both accurate and reliable. By following this step-by-step guide, lab technicians can confidently perform ALT tests while upholding the highest standards of accuracy.
For more information on our ALT testing products or to place an order, visit www.labex.com.pk or contact our team for a consultation on how we can help streamline your lab operations.
Step-by-Step guide on: ALT Test Using Semi auto Chemistry Analyzers